England lock Courtney Lawes has been ruled out of Saturday’s Six Nations home game against Wales with an ankle injury.
The 27-year-old sustained the injury in Northampton’s 20-15 Premiership victory over Saracens on Saturday.
He will be replaced in Eddie Jones’ squad by Ed Slater of Leicester Tigers.
England are top of the Six Nations standings, one point ahead of Wales, with three victories from their three matches played.
Lawes’ Northampton team-mate Luther Burrell also joins England’s 33-man training squad, replacing Marland Yarde of Harlequins, who suffered a shoulder injury during Saturday’s defeat by Sale Sharks.
Bath centre Ollie Devoto has recovered from concussion to make the squad, while Leicester’s Manu Tuilagi, who has not played for England since 2014, is also included.
However, Leicester coach Richard Cockerill does not believe Tuilagi is ready to return to Test rugby yet.
Speaking after the 24-year-old scored his first Premiership try of the season during the 31-27 win over Exeter on Sunday, he said: “Manu did some good things today but I don’t think he is ready to play Test rugby.
“Even if you put him on the bench to be an impact player for the last half an hour, what happens if you have to put him on after five minutes?”
England squad in full:
Backs: Mike Brown (Harlequins), Luther Burrell (Northampton), Danny Care (Harlequins), Elliot Daly (Wasps), Ollie Devoto (Bath), Owen Farrell (Saracens), George Ford (Bath), Alex Goode (Saracens), Jonathan Joseph (Bath), Jack Nowell (Exeter) Semesa Rokoduguni (Bath), Manu Tuilagi (Leicester), Anthony Watson (Bath), Ben Youngs (Leicester)
Forwards: Kieran Brookes (Northampton), Dan Cole (Leicester), Luke Cowan-Dickie (Exeter), Jack Clifford (Harlequins), Dave Ewers (Exeter), Jamie George (Saracens), Dylan Hartley (Northampton), James Haskell (Wasps), Paul Hill (Northampton), Maro Itoje (Saracens), George Kruis (Saracens), Matt Kvesic (Gloucester), Joe Launchbury (Wasps), Joe Marler (Harlequins), Matt Mullan (Wasps), Chris Robshaw (Harlequins), Ed Slater (Leicester), Billy Vunipola (Saracens), Mako Vunipola (Saracens)